A cell-free ROS-responsive hydrogel/oriented poly(lactide-co-glycolide) hybrid scaffold for reducing inflammation and restoring full-thickness cartilage defectsin vivo.
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| Abstract |
The modulation of inflammation in tissue microenvironment takes an important role in cartilage repair and regeneration. In this study, a novel hybrid scaffold was designed and fabricated by filling a reactive oxygen species (ROS)-scavenging hydrogel (RS Gel) into a radially oriented poly(lactide-co-glycolide) (PLGA) scaffold. The radially oriented PLGA scaffolds were fabricated through a temperature gradient-guided phase separation and freeze-drying method. The RS Gel was formed by crosslinking the mixture of ROS-responsive hyperbranched polymers and biocompatible methacrylated hyaluronic acid (HA-MA). The hybrid scaffolds exhibited a proper compressive modulus, good ROS-scavenging capability, and cell compatibility.In vivotests showed that the hybrid scaffolds significantly regulated inflammation and promoted regeneration of hyaline cartilage after they were implanted into full-thickness cartilage defects in rabbits for 12 w. In comparison with the PLGA scaffolds, the neo-cartilage in the hybrid scaffolds group possessed more deposition of glycosaminoglycans and collagen type II, and were well integrated with the surrounding tissue.
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| Authors | Xinyu Wu, Jie Ding, Peifang Xu, Xue Feng, Zhaoyi Wang, Tong Zhou, Chenxi Tu, Wangbei Cao, Jieqi Xie, Liwen Deng, Liyin Shen, Yang Zhu, Zhongru Gou, Changyou Gao |
| Journal | Biomedical materials (Bristol, England) (Biomed Mater) Vol. 16 Issue 6 (09 14 2021) ISSN: 1748-605X [Electronic] England |
| PMID | 34450597 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | © 2021 IOP Publishing Ltd. |
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