Alpha-asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of
alpha-asarone on
cerebral ischemia-reperfusion (CIR)
stroke has not been reported. The present study aimed to investigate the
neuroprotective effect of
alpha-asarone and the involved mechanisms against CIR
stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the
drug or
drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the
infarct volume was evaluated by Triphenyl Tetrazolium
Chloride (TTC) staining. The neurofunctional recovery and post-
stroke epilepsy were evaluated. Nissl and
Hematoxylin-
Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of
alpha-asarone against the
stroke. The results showed that
alpha-asarone exhibited a desirable
neuroprotective effect, manifested as reducing
infarct volume and post-
stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that
alpha-asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Furthermore,
alpha-asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that
alpha-asarone attenuated the CIR
stroke injury via ameliorating glial activation and autophagy.