cGMP-grade human iPSC-derived retinal photoreceptor precursor cells rescue cone photoreceptor damage in non-human primates.

Abstract

BACKGROUND:

Retinal regenerative therapies hold great promise for the treatment of inherited retinal degenerations (IRDs). Studies in preclinical lower mammal models of IRDs have suggested visual improvement following retinal photoreceptor precursors transplantation, but there is limited evidence on the ability of these transplants to rescue retinal damage in higher mammals. The purpose of this study was to evaluate the therapeutic potential of photoreceptor precursors derived from clinically compliant induced pluripotent stem cells (iPSCs).

METHODS:

Photoreceptor precursors were sub-retinally transplanted into non-human primates (Macaca fascicularis). The cells were transplanted both in naïve and cobalt chloride-induced retinal degeneration models who had been receiving systemic immunosuppression for one week prior to the procedure. Optical coherence tomography, fundus autofluorescence imaging, electroretinography, ex vivo histology and immunofluorescence staining were used to evaluate retinal structure, function and survival of transplanted cells.

RESULTS:

There were no adverse effects of iPSC-derived photoreceptor precursors on retinal structure or function in naïve NHP models, indicating good biocompatibility. In addition, photoreceptor precursors injected into cobalt chloride-induced retinal degeneration NHP models demonstrated an ability both to survive and to mature into cone photoreceptors at 3 months post-transplant. Optical coherence tomography showed restoration of retinal ellipsoid zone post-transplantation.

CONCLUSIONS:

These findings demonstrate the safety and therapeutic potential of clinically compliant iPSC-derived photoreceptor precursors as a cell replacement source for future clinical trials.

• Authors: Swathi Lingam, Zengping Liu, Binxia Yang, Wendy Wong, Bhav Harshad Parikh, Jun Yi Ong, Debbie Goh, Daniel Soo Lin Wong, Queenie Shu Woon Tan, Gavin S W Tan, Graham E Holder, Kakkad Regha, Veluchamy Amutha Barathi, Walter Hunziker, Gopal Lingam, Xianmin Zeng, Xinyi Su
• Journal: Stem cell research & therapy (Stem Cell Res Ther) Vol. 12 Issue 1 Pg. 464 (08 19 2021) ISSN: 1757-6512 [Electronic] England
• PMID: 34412697 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Author(s).

Topics

• Animals
• Humans
• Induced Pluripotent Stem Cells
• Photoreceptor Cells, Vertebrate
• Primates
• Retinal Cone Photoreceptor Cells
• Retinal Degeneration (therapy)