Abstract |
Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a chronic inflammatory process often associated with comorbid asthma. In this study, we analyzed the transcriptomes of single T helper (TH) cells from nasal polyps of patients with CRSwNP and validated these findings using multiparameter flow cytometry. Polyp tissue contained suppressive T regulatory (Treg) cells, TH2 cells, type 2 innate lymphoid cells, and three transcriptionally distinct subsets of cytotoxic CD4+ T cells (CD4+ CTL). GATA3 expression was a feature of polyp Treg cells, whereas TH2 cells highly expressed TCN1, CD200R, and HPGDS and were enriched for genes involved in lipid metabolism. Only a portion of polyp TH2 cells expressed the prostaglandin D2 receptor CRTH2, whereas a subpopulation of CD109+CRTH2- TH2 cells expressed mRNA for common inhibitor receptors including LAG3 and TIM3 and produced IL-10. Together, we resolved the complexity of TH cells in patients with CRSwNP, identifying several distinct clusters of CD4+ CTL and a population of CD109+CRTH2- TH2 cells with putative regulatory potential.
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Authors | Junjie Ma, Christopher A Tibbitt, Susanna Kumlien Georén, Murray Christian, Ben Murrell, Lars-Olaf Cardell, Claus Bachert, Jonathan M Coquet |
Journal | Science immunology
(Sci Immunol)
Vol. 6
Issue 62
(08 13 2021)
ISSN: 2470-9468 [Electronic] United States |
PMID | 34389612
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. |
Chemical References |
- Antigens, CD
- CD109 protein, human
- GPI-Linked Proteins
- IL10 protein, human
- Neoplasm Proteins
- Interleukin-10
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Topics |
- Antigens, CD
(immunology)
- CD4-Positive T-Lymphocytes
(immunology, pathology)
- GPI-Linked Proteins
(immunology)
- Humans
- Interleukin-10
(immunology)
- Nasal Polyps
(immunology, pathology)
- Neoplasm Proteins
(immunology)
- Single-Cell Analysis
- T-Lymphocytes, Cytotoxic
(immunology, pathology)
- Th2 Cells
(immunology, pathology)
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