HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Single-cell analysis pinpoints distinct populations of cytotoxic CD4+ T cells and an IL-10+CD109+ TH2 cell population in nasal polyps.

Abstract
Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by a chronic inflammatory process often associated with comorbid asthma. In this study, we analyzed the transcriptomes of single T helper (TH) cells from nasal polyps of patients with CRSwNP and validated these findings using multiparameter flow cytometry. Polyp tissue contained suppressive T regulatory (Treg) cells, TH2 cells, type 2 innate lymphoid cells, and three transcriptionally distinct subsets of cytotoxic CD4+ T cells (CD4+ CTL). GATA3 expression was a feature of polyp Treg cells, whereas TH2 cells highly expressed TCN1, CD200R, and HPGDS and were enriched for genes involved in lipid metabolism. Only a portion of polyp TH2 cells expressed the prostaglandin D2 receptor CRTH2, whereas a subpopulation of CD109+CRTH2- TH2 cells expressed mRNA for common inhibitor receptors including LAG3 and TIM3 and produced IL-10. Together, we resolved the complexity of TH cells in patients with CRSwNP, identifying several distinct clusters of CD4+ CTL and a population of CD109+CRTH2- TH2 cells with putative regulatory potential.
AuthorsJunjie Ma, Christopher A Tibbitt, Susanna Kumlien Georén, Murray Christian, Ben Murrell, Lars-Olaf Cardell, Claus Bachert, Jonathan M Coquet
JournalScience immunology (Sci Immunol) Vol. 6 Issue 62 (08 13 2021) ISSN: 2470-9468 [Electronic] United States
PMID34389612 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
Chemical References
  • Antigens, CD
  • CD109 protein, human
  • GPI-Linked Proteins
  • IL10 protein, human
  • Neoplasm Proteins
  • Interleukin-10
Topics
  • Antigens, CD (immunology)
  • CD4-Positive T-Lymphocytes (immunology, pathology)
  • GPI-Linked Proteins (immunology)
  • Humans
  • Interleukin-10 (immunology)
  • Nasal Polyps (immunology, pathology)
  • Neoplasm Proteins (immunology)
  • Single-Cell Analysis
  • T-Lymphocytes, Cytotoxic (immunology, pathology)
  • Th2 Cells (immunology, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: