Solute carrier family 12 member 8 (SLC12A8) is a potential biomarker and related to tumor immune cell infiltration in bladder cancer.

Abstract

The solute carrier family has been reported to play critical roles in the progression of several cancers; however, the relationship between solute carrier family 12 member 8 (SLC12A8) and bladder cancer (BC) has not been clearly confirmed. This study explores the prognostic value of SLC12A8 for BC and its correlation with immune cell infiltration. We found that the expression of SLC12A8 mRNA was significantly overexpressed in BC tissues compared with noncancerous tissues in multiple public databases, and the result was validated using real-time PCR and immunohistochemistry (IHC). The Kaplan-Meier method and Cox proportional hazards models were used to evaluate the prognostic value of SLC12A8 for BC. The high expression of SLC12A8 led to a shorter overall survival time and was an unfavorable prognostic biomarker for BC. The mechanisms of SLC12A8 promoting tumorigenesis were investigated by Gene Set Enrichment Analysis (GSEA). Moreover, the correlations of SLC12A8 expression with the tumor-infiltrating immune cells (TICs) in BC were explored using TIMER 2.0 and CIBERSORT. SLC12A8 was associated with CD4+ T cells, dendritic cells, neutrophils, and macrophages infiltration. The expression of SLC12A8 was positively correlated with crucial immune checkpoint molecules. In conclusion, SLC12A8 might be an unfavorable prognostic biomarker in BC related to tumor immune cell infiltration.

• Authors: Qian Zhang, Yunen Liu, Peng Chen, Xiuyun Shi, Ying Liu, Lin Shi, Peifang Cong, Shun Mao, Cangci Tong, Cheng Du, Mingxiao Hou
• Journal: Bioengineered (Bioengineered) Vol. 12 Issue 1 Pg. 4946-4961 (12 2021) ISSN: 2165-5987 [Electronic] United States
• PMID: 34365894 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• SLC12A8 protein, human
• Sodium-Potassium-Chloride Symporters

Topics

• Aged
• Aged, 80 and over
• Biomarkers, Tumor
• Cell Line, Tumor
• Databases, Genetic
• Female
• Humans
• Male
• Middle Aged
• Prognosis
• Sodium-Potassium-Chloride Symporters (genetics, immunology, metabolism)
• Urinary Bladder (pathology)
• Urinary Bladder Neoplasms (genetics, immunology, mortality, pathology)