HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Kidney organoids generated from erythroid progenitors cells of patients with autosomal dominant polycystic kidney disease.

AbstractBACKGROUND:
Kidney organoids have been broadly obtained from commercially available induced pluripotent stem cells (iPSCs); however, it has been a great challenge to efficiently produce renal organoid models from patients with autosomal dominant polycystic kidney disease (ADPKD) that recapitulate both embryogenesis and the mechanisms of cystogenesis.
METHODS:
Blood erythroid progenitors (EPs) from two ADPKD patients and one healthy donor (HC) was used as a comparative control to normalize the many technical steps for reprogramming EPs and for the organoids generation. EPs were reprogrammed by an episomal vector into iPSCs, which were differentiated into renal tubular organoids and then stimulated by forskolin to induce cysts formation.
RESULTS:
iPSCs derived from EPs exhibited all characteristics of pluripotency and were able to differentiate into all three germ layers. 3D tubular organoids were generated from single cells after 28 days in Matrigel. HC and ADPKD organoids did not spontaneously form cysts, but upon forskolin stimulation, cysts-like structures were observed in the ADPKD organoids but not in the HC-derived organoids.
CONCLUSION:
The findings of this study showed that kidney organoids were successfully generated from the blood EP cells of ADPKD patients and a healthy control donor. This approach should contribute as a powerful tool for embryonic kidney development model, which is able to recapitulate the very early pathophysiological mechanisms involved in cytogenesis.
AuthorsRoberta Facioli, Fernando Henrique Lojudice, Ana Carolina Anauate, Edgar Maquigussa, José Luiz Nishiura, Ita Pfeferman Heilberg, Mari Cleide Sogayar, Mirian Aparecida Boim
JournalPloS one (PLoS One) Vol. 16 Issue 8 Pg. e0252156 ( 2021) ISSN: 1932-6203 [Electronic] United States
PMID34339420 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Topics
  • Erythroid Precursor Cells (metabolism, pathology)
  • Female
  • Humans
  • Induced Pluripotent Stem Cells (metabolism, pathology)
  • Kidney (metabolism, pathology)
  • Organoids (metabolism, pathology)
  • Polycystic Kidney, Autosomal Dominant (metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: