Abstract |
Epstein-Barr virus ( EBV) infection is an established cause of nasopharyngeal carcinoma (NPC) and is involved in a variety of malignant phenotypes, including tumor immune escape. EBV can encode a variety of circular RNAs ( circRNA), however, little is known regarding the biological functions of these circRNAs in NPC. In this study, EBV-encoded circBART2.2 was found to be highly expressed in NPC where it upregulated PD-L1 expression and inhibited T-cell function in vitro and in vivo. circBART2.2 promoted transcription of PD-L1 by binding the helicase domain of RIG-I and activating transcription factors IRF3 and NF-κB, resulting in tumor immune escape. These results elucidate the biological function of circBART2.2, explain a novel mechanism of immune escape caused by EBV infection, and provide a new immunotherapy target for treating NPC. SIGNIFICANCE: This work demonstrates that circBART2.2 binding to RIG-I is essential for the regulation of PD-L1 and subsequent immune escape in nasopharyngeal carcinoma.
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Authors | Junshang Ge, Jie Wang, Fang Xiong, Xianjie Jiang, Kunjie Zhu, Yian Wang, Yongzhen Mo, Zhaojian Gong, Shanshan Zhang, Yi He, Xiayu Li, Lei Shi, Can Guo, Fuyan Wang, Ming Zhou, Bo Xiang, Yong Li, Guiyuan Li, Wei Xiong, Zhaoyang Zeng |
Journal | Cancer research
(Cancer Res)
Vol. 81
Issue 19
Pg. 5074-5088
(10 01 2021)
ISSN: 1538-7445 [Electronic] United States |
PMID | 34321242
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | ©2021 The Authors; Published by the American Association for Cancer Research. |
Chemical References |
- B7-H1 Antigen
- CD274 protein, human
- NF-kappa B
- RNA, Circular
- RNA, Viral
- Receptors, Immunologic
- RIGI protein, human
- DEAD Box Protein 58
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Topics |
- Animals
- B7-H1 Antigen
(genetics, metabolism)
- Cell Line, Tumor
- Cytotoxicity, Immunologic
(genetics)
- DEAD Box Protein 58
(genetics, metabolism)
- Disease Models, Animal
- Disease Susceptibility
- Epstein-Barr Virus Infections
(complications, virology)
- Gene Expression Regulation, Neoplastic
- Herpesvirus 4, Human
(genetics)
- Humans
- Mice
- NF-kappa B
(metabolism)
- Nasopharyngeal Carcinoma
(etiology, metabolism, pathology)
- Protein Binding
- RNA Interference
- RNA, Circular
(genetics)
- RNA, Viral
(genetics)
- Receptors, Immunologic
(genetics, metabolism)
- Tumor Escape
(genetics)
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