Paeoniflorin ameliorates oxidase stress in Glutamate-stimulated SY5Y and prenatally stressed female offspring through Nrf2/HO-1 signaling pathway.

Abstract

BACKGROUND:

Prenatal stress (PS) can cause brain retardation, reduce the learning and memory ability of the offspring and significantly increase the incidence of depression in offspring. Paeoniflorin (PF), a kind of monoterpenoid glycoside, is one of the main active ingredients of Chinese Medicine Paeonia lactiflora Pall, has anti-inflammation and potential neuroprotective effects. However, few reports have shown that the neuroprotective effects of PF are exerted through ameliorating Glutamate toxicity in vivo and in vitro.

METHODS:

Here, we used a prenatal restraint stress model and Glu-induced excitotoxic neurotoxicity in SH-SY5Y cells to study the effects of PF.

RESULTS:

Our results showed that PF can ameliorate learning and memory impairments and increases the density of hippocampal neurons, typical Golgi-positive pyramidal cells, and neuronal Neurogranin (Ng) expression in PS rat offspring. Furthermore, PF can significantly up-regulate the decrease of Glu-induced SH-SY5Y cell viability. At the same time, PF can significantly reduce apoptosis, ROS, NO levels, and intracellular Ca2+ concentration, and significantly inhibit the increase of mitochondrial membrane potential. Besides, PF significantly increased the expression of Nrf2 and iNOS, decreased p-JNK/JNK, p-P38/P38, Bax/Bcl-2, active-caspase-3, and active-caspase-9.

CONCLUSIONS:

Our results demonstrate that PF may be an effective treatment in preventing the development of PS-induced learning and memory impairment and have therapeutic potential in Glu-related neurological diseases.

• Authors: Xing Wang, Jin Cheng Hao, Bo Shang, Kai Lin Yang, Xiao Zhou He, Zhao Liang Wang, Hui Ling Jing, Yan Jun Cao
• Journal: Journal of affective disorders (J Affect Disord) Vol. 294 Pg. 189-199 (11 01 2021) ISSN: 1573-2517 [Electronic] Netherlands
• PMID: 34298225 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Glucosides
• Monoterpenes
• NF-E2-Related Factor 2
• Neuroprotective Agents
• Nfe2l2 protein, rat
• peoniflorin
• Glutamic Acid
• Oxidoreductases
• Heme Oxygenase (Decyclizing)
• Hmox1 protein, rat

Topics

• Animals
• Apoptosis
• Female
• Glucosides
• Glutamic Acid
• Heme Oxygenase (Decyclizing)
• Monoterpenes (pharmacology)
• NF-E2-Related Factor 2 (metabolism, pharmacology)
• Neuroprotective Agents (pharmacology)
• Oxidative Stress
• Oxidoreductases
• Pregnancy
• Rats
• Signal Transduction