Abstract | BACKGROUND: Various clinical patterns based on routes of sensitization and sensitized allergens are reported in adult-onset IgE-mediated wheat allergy. There is still a paucity of data on IgE-bound wheat allergen profiles in wheat challenge-proven adult-onset wheat allergic cases. Therefore, we aim to identify the major sensitized allergens in Thai adult-onset wheat allergic patients whose first symptom occurred after the age of 18 years despite previous tolerance. METHODS: This cross-sectional pilot study recruited patients from the Thai Adult-onset IgE-mediated Wheat Allergy Cohort (TAWAC). The sera of patients with mostly challenge-proven cases were selected for allergen study, including ImmunoCAP and IgE-bound gliadins along with glutenins profiles. The IgE-bound proteins were identified by liquid chromatography-tandem mass spectrophotometry (LC-MS/MS). Direct binding of IgE to recombinant gliadin and glutenin was performed to confirm the results of immunoblot and LC-MS/MS. RESULTS: CONCLUSION:
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Authors | Surapon Piboonpocanun, Torpong Thongngarm, Chamard Wongsa, Punchama Pacharn, Onrapak Reamtong, Mongkhon Sompornrattanaphan |
Journal | Journal of asthma and allergy
(J Asthma Allergy)
Vol. 14
Pg. 907-917
( 2021)
ISSN: 1178-6965 [Print] New Zealand |
PMID | 34295166
(Publication Type: Journal Article)
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Copyright | © 2021 Piboonpocanun et al. |