The Host Protein Aquaporin-9 is Required for Efficient Plasmodium falciparum Sporozoite Entry into Human Hepatocytes.

Abstract

Hepatocyte invasion by Plasmodium sporozoites represents a promising target for innovative antimalarial therapy, but the molecular events mediating this process are still largely uncharacterized. We previously showed that Plasmodium falciparum sporozoite entry into hepatocytes strictly requires CD81. However, CD81-overexpressing human hepatoma cells remain refractory to P. falciparum infection, suggesting the existence of additional host factors necessary for sporozoite entry. Here, through differential transcriptomic analysis of human hepatocytes and hepatoma HepG2-CD81 cells, the transmembrane protein Aquaporin-9 (AQP9) was found to be among the most downregulated genes in hepatoma cells. RNA silencing showed that sporozoite invasion of hepatocytes requires AQP9 expression. AQP9 overexpression in hepatocytes increased their permissiveness to P. falciparum. Moreover, chemical disruption with the AQP9 inhibitor phloretin markedly inhibited hepatocyte infection. Our findings identify AQP9 as a novel host factor required for P. falciparum sporozoite hepatocyte-entry and indicate that AQP9 could be a potential therapeutic target.

• Authors: Nadia Amanzougaghene, Shahin Tajeri, Samir Yalaoui, Audrey Lorthiois, Valérie Soulard, Audrey Gego, Armelle Rametti, Véronica Risco-Castillo, Alicia Moreno, Maurel Tefit, Geert-Jan van Gemert, Robert W Sauerwein, Jean-Christophe Vaillant, Philippe Ravassard, Jean-Louis Pérignon, Patrick Froissard, Dominique Mazier, Jean-François Franetich
• Journal: Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 11 Pg. 704662 ( 2021) ISSN: 2235-2988 [Electronic] Switzerland
• PMID: 34268141 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Amanzougaghene, Tajeri, Yalaoui, Lorthiois, Soulard, Gego, Rametti, Risco-Castillo, Moreno, Tefit, van Gemert, Sauerwein, Vaillant, Ravassard, Pérignon, Froissard, Mazier and Franetich.

Chemical References

• AQP9 protein, human
• Aquaporins
• Protozoan Proteins
• Tetraspanin 28

Topics

• Animals
• Aquaporins
• Hepatocytes (metabolism)
• Humans
• Plasmodium falciparum
• Protozoan Proteins (genetics, metabolism)
• Sporozoites (metabolism)
• Tetraspanin 28 (metabolism)