The
severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) 3CL
protease is a promising target for inhibition of viral replication by interaction with a
cysteine residue (Cys145) at its catalytic site.
Dalcetrapib exerts its
lipid-modulating effect by binding covalently to
cysteine 13 of a
cholesteryl ester transfer protein. Because 12 free
cysteine residues are present in the 3CL
protease, we investigated the potential of
dalcetrapib to inhibit 3CL
protease activity and SARS-CoV-2 replication. Molecular docking investigations suggested that
dalcetrapib-
thiol binds to the catalytic site of the 3CL
protease with a delta G value of -8.5 kcal/mol.
Dalcetrapib inhibited both 3CL
protease activity in vitro and viral replication in Vero E6 cells with IC50 values of 14.4 ± 3.3 μM and an EC50 of 17.5 ± 3.5 μM (mean ± SD). Near-complete inhibition of
protease activity persisted despite 1000-fold dilution after ultrafiltration with a nominal
dalcetrapib-
thiol concentration of approximately 100 times below the IC50 of 14.4 μM, suggesting stable
protease-drug interaction. The inhibitory effect of
dalcetrapib on the SARS-CoV-2 3CL
protease and viral replication warrants its clinical evaluation for the treatment of
COVID-19.