Evaluation of the hemostatic effect of a combination of hemostatic agents and fibrin glue in a rabbit venous hemorrhage model.

Abstract

BACKGROUND:

In neurosurgery, it is important to use local hemostatic agents. We have explored a more powerful method of hemostasis by the combination of commercially available hemostatic agents with fibrin glue in the hopes of synergistic effects.

METHOD:

A bleeding model was constructed by puncturing the rabbit posterior vena cava with a needle. After applying the sample to the bleeding point, compression was performed for 10 s. If temporary hemostasis was achieved after pressure release, a 30 s wash was performed to confirm that ultimate hemostasis was achieved. Up to three hemostasis attempts were performed on the same bleeding point until hemostasis was achieved, and the number of attempts required for hemostasis was counted. If hemostasis was not achieved after three attempts, it was counted as four times. Four groups were evaluated: (1) gelatin sponge alone, (2) gelatin sponge + fibrin glue, (3) oxidized cellulose alone, and (4) oxidized cellulose + fibrin glue; each group was tested 16 times.

RESULTS:

The median value (range minimum value-maximum value) of the number of hemostatic attempts in Group 1 to Group 4 was 3 (1-4), 1 (1-1), 4 (4-4), and 4 (2-4). In Group 2, there were two test exclusions owing to deviations of the test procedure.

CONCLUSIONS:

The compatibility of gelatin sponge and fibrin glue was very good, with a very strong and rapid hemostatic effect compared to other methods, showed its usefulness. This combination method may be effective for a variety of venous hemorrhages in neurosurgery.

• Authors: Katsumi Takizawa, Daiki Okazaki, Yoshitaka Takegawa, Yuki Koga, Masataka Sagata, Kenichi Michishita, Noriko Shinya
• Journal: BMC neurology (BMC Neurol) Vol. 21 Issue 1 Pg. 270 (Jul 07 2021) ISSN: 1471-2377 [Electronic] England
• PMID: 34233632 (Publication Type: Journal Article)

Chemical References

• Fibrin Tissue Adhesive
• Hemostatics

Topics

• Animals
• Disease Models, Animal
• Fibrin Tissue Adhesive (administration & dosage, pharmacology)
• Hemorrhage
• Hemostasis (drug effects)
• Hemostatics (administration & dosage, pharmacology)
• Rabbits