The Antigenicity of Epidemic SARS-CoV-2 Variants in the United Kingdom.

Abstract

To determine whether the neutralization activity of monoclonal antibodies, convalescent sera and vaccine-elicited sera was affected by the top five epidemic SARS-CoV-2 variants in the UK, including D614G+L18F+A222V, D614G+A222V, D614G+S477N, VOC-202012/01(B.1.1.7) and D614G+69-70del+N439K, a pseudovirus-neutralization assay was performed to evaluate the relative neutralization titers against the five SARS-CoV-2 variants and 12 single deconvolution mutants based on the variants. In this study, 18 monoclonal antibodies, 10 sera from convalescent COVID-19 patients, 10 inactivated-virus vaccine-elicited sera, 14 mRNA vaccine-elicited sera, nine RBD-immunized mouse sera, four RBD-immunized horse sera, and four spike-encoding DNA-immunized guinea pig sera were tested and analyzed. The N501Y, N439K, and S477N mutations caused immune escape from nine of 18 mAbs. However, the convalescent sera, inactivated virus vaccine-elicited sera, mRNA vaccine-elicited sera, spike DNA-elicited sera, and recombinant RBD protein-elicited sera could still neutralize these variants (within three-fold changes compared to the reference D614G variant). The neutralizing antibody responses to different types of vaccines were different, whereby the response to inactivated-virus vaccine was similar to the convalescent sera.

• Authors: Jiajing Wu, Li Zhang, Yue Zhang, Haixin Wang, Ruxia Ding, Jianhui Nie, Qianqian Li, Shuo Liu, Yongxin Yu, Xiaoming Yang, Kai Duan, Xiaowang Qu, Youchun Wang, Weijin Huang
• Journal: Frontiers in immunology (Front Immunol) Vol. 12 Pg. 687869 ( 2021) ISSN: 1664-3224 [Electronic] Switzerland
• PMID: 34220844 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Wu, Zhang, Zhang, Wang, Ding, Nie, Li, Liu, Yu, Yang, Duan, Qu, Wang and Huang.

Chemical References

• Antibodies, Monoclonal
• Antibodies, Neutralizing
• Antibodies, Viral
• COVID-19 Vaccines
• Spike Glycoprotein, Coronavirus
• spike protein, SARS-CoV-2

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Neutralizing (immunology)
• Antibodies, Viral (immunology)
• COVID-19 (immunology, therapy)
• COVID-19 Vaccines (immunology)
• Cell Line
• HEK293 Cells
• Humans
• Immunization, Passive
• Mice
• Neutralization Tests (methods)
• SARS-CoV-2 (immunology)
• Spike Glycoprotein, Coronavirus (immunology)
• United Kingdom
• Vaccination
• COVID-19 Serotherapy