Developing fluoromodule-based probes for in vivo monitoring the bacterial infections and antibiotic responses.

Abstract

Recently, antibiotic resistant has become a serious public health concern, which warrants new generations of antibiotics to be developed. Pharmacodynamic evaluation is crucial in drug discovery processes. Despite numerous advanced imaging systems are available nowadays, technologies for the sensitive in vivo diagnosis of bacterial infections and direct visualization of drug efficacy are yet to be developed. In this study, we have developed novel near-infrared (NIR) fluorogenic probes. These probes are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP). We established the in vivo bacterial infection model using FAP_dH6.2 recombinantly expressed E. coli and applied this NIR fluoromodule-based system for diagnosing bacterial infections and monitoring disease progressions and its responses to a type of antibiotics through classic mechanism of membrane lysis. This NIR fluoromodule-based system will discover new information on bacterial infections and identify newer antibacterial entities.

• Authors: Xiang Wang, Qinghua Wang, Qingyang Zhang, Xiaowan Han, Shengnan Xu, Dali Yin, Hai-Yu Hu
• Journal: Talanta (Talanta) Vol. 233 Pg. 122610 (Oct 01 2021) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 34215094 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Fluorescent Dyes
• Proteins

Topics

• Anti-Bacterial Agents (pharmacology)
• Bacterial Infections (diagnosis, drug therapy)
• Escherichia coli (genetics)
• Fluorescent Dyes
• Humans
• Proteins