Abstract | INTRODUCTION: METHODS: Exosomes were extracted using the ExoQuick Precipitation Kit (System Biosciences, Mountain View, CA, USA). The levels of circ_0081234, miR-1 and mitogen-activated protein kinase kinase kinase 1 (MAP 3 K1) were examined using a quantitative real-time polymerase chain reaction or western blotting. Cell migration and invasion were evaluated via a transwell assay. The protein levels of N-cadherin, vimentin and E-cadherin were detected by western blotting. The interaction between miR-1 and circ_0081234 or MAP 3 K1 was verified via a dual- luciferase reporter assay and an RNA pull-down assay. RESULTS: The circ_0081234 level was increased in PCa tissues with spinal metastasis in comparison to primary PCa tissues without spinal metastasis. Exosomal circ_0081234 promoted the migration, invasion and epithelial-mesenchymal transition of PCa cells. Knockdown of circ_0081234 blocked PCa cell progression by regulating miR-1. In addition, miR-1 overexpression suppressed PCa cell progression by repressing MAP 3 K1. Moreover, circ_0081234 increased MAP 3 K1 level via sponging miR-1. Depletion of circ_0081234 inhibited tumor growth in vivo. CONCLUSIONS: Exosomal circ_0081234 promoted migration, invasion and epithelial-mesenchymal transition of PCa cells by regulating the miR-1/MAP 3 K1 axis.
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Authors | Guangyao Zhang, Yibin Liu, Jianhui Yang, Haiming Wang, Zhengwei Xing |
Journal | The journal of gene medicine
(J Gene Med)
Vol. 24
Issue 8
Pg. e3376
(08 2022)
ISSN: 1521-2254 [Electronic] England |
PMID | 34191363
(Publication Type: Journal Article)
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Copyright | © 2021 John Wiley & Sons, Ltd. |
Chemical References |
- MIRN1 microRNA, human
- MicroRNAs
- RNA, Circular
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Topics |
- Cell Line, Tumor
- Cell Proliferation
(genetics)
- Humans
- Male
- MicroRNAs
(metabolism)
- Prostatic Neoplasms
(genetics)
- RNA, Circular
(genetics)
- Spinal Neoplasms
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