Sulforaphane protects against oxidative stress‑induced apoptosis via activating SIRT1 in mouse osteoarthritis.

Abstract

Osteoarthritis (OA), the most common form of human joint disease, is characterized by progressive degeneration of the articular cartilage, synovitis and subchondral osteoporosis. Chondrocyte apoptosis is the primary pathogenic mechanism of OA and is considered to be a potential therapeutic target. Sulforaphane (SFN), a dietary isothiocyanate obtained from cruciferous vegetables, has been reported to exert an anti‑apoptotic effect by activating sirtuin 1 (SIRT1). To the best of our knowledge, however, the effects of SFN on apoptotic responses in OA have not been reported. In the present study, SFN was shown to significantly inhibit chondrocyte apoptosis while enhancing expression levels of SIRT1 in a H2O2‑induced OA mouse model. The anti‑apoptotic effect of SFN was reversed by SIRT1 small interfering RNA, implying that SIRT1 exerted a protective role against the effect of SFN on chondrocytes. The expression levels of C/EBP homologous protein, 78‑kDa glucose regulated protein, Bax, Bcl‑2 and cleaved caspase 3 were found to be downregulated in SFN‑treated mice. Furthermore, SFN ameliorated cartilage degradation in the OA mouse model. These findings indicate that SFN exerted an anti‑apoptotic effect on chondrocytes and ameliorated OA in vivo by activating the SIRT1 signaling pathway.

• Authors: Mangmang Chen, Lipeng Huang, Yangxun Lv, Liubing Li, Qirong Dong
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 24 Issue 2 (Aug 2021) ISSN: 1791-3004 [Electronic] Greece
• PMID: 34184072 (Publication Type: Journal Article)

Chemical References

• Isothiocyanates
• Protective Agents
• Sulfoxides
• Hydrogen Peroxide
• Sirt1 protein, mouse
• Sirtuin 1
• sulforaphane

Topics

• Animals
• Apoptosis (drug effects)
• Cell Survival (drug effects)
• Chondrocytes (cytology, drug effects, metabolism)
• Disease Models, Animal
• Endoplasmic Reticulum Stress (drug effects)
• Female
• Gene Silencing
• Hydrogen Peroxide (toxicity)
• Isothiocyanates (pharmacology, therapeutic use)
• Male
• Mice, Inbred C57BL
• Osteoarthritis (etiology, metabolism, prevention & control)
• Oxidative Stress (drug effects)
• Primary Cell Culture
• Protective Agents (pharmacology, therapeutic use)
• Sirtuin 1 (genetics, metabolism)
• Sulfoxides (pharmacology, therapeutic use)
• Tibial Meniscus Injuries (complications)
• Mice