Polymorphism in miRNA target sites of CEP-63 and CEP-152 ring complex influences expression of CEP genes and favors tumorigenesis in glioma.

Abstract

Purpose: The present study was designed to screen the genetic polymorphisms and expression profiling of CEP-152 and CEP-63 genes in brain tumor patients. Methods: The amplification refractory mutation system PCR technique (ARMS-PCR) was used for mutation analysis using 300 blood samples of brain tumor patients and 300 overtly healthy controls. For expression analysis, 150 brain tumor tissue samples along with adjacent uninvolved/normal tissues (controls) were collected. Results: A significantly higher frequency of the mutant genotype of the CEP-152 single nucleotide polymorphism (rs2169757) and CEP-63 single nucleotide polymorphisms (rs9809619 and rs13060247) was observed in patients versus overtly healthy controls. The authors' results showed highly significant deregulation of CEP-152 (p < 0.0001) and CEP-63 (p < 0.0001) in glioma/meningioma tumor tissues versus adjacent normal tissue. Conclusion: The present study showed that variations in CEP-152 and CEP-63 genes were associated with an increased risk of brain tumor.

• Authors: Ishrat Mahjabeen, Yusra Maqsood, Ramsha Abbasi, Malik Waqar Ahmed, Mahmood Akhtar Kayani
• Journal: Future oncology (London, England) (Future Oncol) Vol. 17 Issue 25 Pg. 3355-3372 (Sep 2021) ISSN: 1744-8301 [Electronic] England
• PMID: 34156311 (Publication Type: Journal Article, Observational Study)

Chemical References

• 3' Untranslated Regions
• CEP152 protein, human
• CEP63 protein, human
• Cell Cycle Proteins
• MicroRNAs

Topics

• 3' Untranslated Regions (genetics)
• Adult
• Brain (pathology, surgery)
• Brain Neoplasms (blood, epidemiology, genetics, surgery)
• Carcinogenesis (genetics)
• Case-Control Studies
• Cell Cycle Proteins (genetics)
• DNA Mutational Analysis
• Female
• Follow-Up Studies
• Gene Expression Regulation, Neoplastic
• Genetic Predisposition to Disease
• Glioma (blood, epidemiology, genetics, surgery)
• Humans
• Male
• MicroRNAs (metabolism)
• Polymorphism, Single Nucleotide
• Risk Assessment (methods, statistics & numerical data)