Inhibition of dynamin-related protein 1 ameliorates the mitochondrial ultrastructure via PINK1 and Parkin in the mice model of Parkinson's disease.

Abstract

Parkinson's disease (PD) is the prevalent neurodegenerative disorder characterized by the degeneration of the nigrostriatal neurons. Dynamin-related protein 1 (Drp1) is a key regulator mediating mitochondrial fission and affecting mitophagy in neurons. It has been reported that the inhibition of Drp1 may be beneficial to PD. However, the role of Drp1 and mitophagy in PD remains elusive. Therefore, in this research, we investigated the role of Drp1 and the underlying mechanisms in the mice model of PD. We used the dynasore, a GTPase inhibitor, to inhibit the expression of Drp1. We found that inhibition of Drp1 could ameliorate the motor deficits and the expression of tyrosine hydroxylase in the mice of the PD model. But Drp1 inhibition did not affect mitochondria number and morphological parameters. Moreover, suppression of Drp1 up-regulated the mitochondrial expressions of PINK1 and Parkin while not affected the expressions of NIX and BNIP3. Conclusively, our findings suggest that the inhibition of Drp1 ameliorated the mitochondrial ultrastructure at least via regulating PINK1 and Parkin in the mice of the PD model. This study also implicates that inhibition of Drp1 might impact mitophagy and recover mitochondrial homeostasis in PD.

• Authors: Si-Tong Feng, Zhen-Zhen Wang, Yu-He Yuan, Xiao-Le Wang, Zhen-Yu Guo, Jing-Hong Hu, Xu Yan, Nai-Hong Chen, Yi Zhang
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 907 Pg. 174262 (Sep 15 2021) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 34146589 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Ubiquitin-Protein Ligases
• parkin protein
• Dynamins

Topics

• Animals
• Dynamins
• Humans
• Mice
• Parkinson Disease
• Ubiquitin-Protein Ligases