Abstract | BACKGROUND: METHODS: We used pre-treatment EDTA plasma from 80 ESCC patients (44% clinical stages I and II) and 80 cancer-free control individuals within the Hospital-based Epidemiologic Research Program at Aichi Cancer Center. Levels of 184 biomarkers were measured by high-throughput multiplexed proximity extension assays using Olink's Proseek Cell Regulation and Immuno-Oncology Panels. ESCC odds ratios (OR) per quantile (based on two to four categories) of each biomarker were calculated by unconditional logistic regression models, adjusted for age, sex, cigarette smoking and alcohol consumption. Correlations among continuous biomarker levels were assessed by Spearman's rank correlation. All statistical tests were two-sided with p values < 0.05 considered as significant. Given the exploratory nature of the study, we did not adjust for multiple comparisons. RESULTS: Seven proteins were undetectable in nearly all samples. Of the remaining 177 evaluable biomarkers, levels of cluster of differentiation 40 (CD40, per quartile OR 1.64; p trend = 0.018), syntaxin 16 (STX16, per quartile OR 1.63; p trend = 0.008), heme oxygenase 1 (per quartile OR 1.59; p trend = 0.014), and γ- secretase activating protein (GSAP, per quartile OR 1.47; p trend = 0.036) were significantly associated with ESCC. Amongst these significant markers, levels of CD40, STX16, and GSPA were moderately correlated (Rho coefficients 0.46-0.55; p < 0.05). CONCLUSION: Our case-control study expands the range of inflammation and immune molecules associated with ESCC. These novel findings warrant replication and functional characterization.
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Authors | M Constanza Camargo, Minkyo Song, Hidemi Ito, Isao Oze, Yuriko N Koyanagi, Yumiko Kasugai, Charles S Rabkin, Keitaro Matsuo |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 147
Issue 10
Pg. 2885-2892
(Oct 2021)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 34128078
(Publication Type: Journal Article)
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Copyright | © 2021. This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply. |
Chemical References |
- Biomarkers, Tumor
- Inflammation Mediators
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Topics |
- Aged
- Alcohol Drinking
(adverse effects)
- Biomarkers, Tumor
(blood)
- Case-Control Studies
- Cigarette Smoking
(adverse effects)
- Esophageal Neoplasms
(blood, etiology, pathology)
- Esophageal Squamous Cell Carcinoma
(blood, etiology, pathology)
- Female
- Follow-Up Studies
- Humans
- Immunity
(immunology)
- Inflammation
(complications)
- Inflammation Mediators
(blood)
- Male
- Prognosis
- Risk Factors
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