Protein Arrays for the Identification of Seroreactive Protein Markers for Infectious Diseases.

Abstract

The protein array is a powerful platform to study humoral responses to infectious agents using small sample volumes [<3 μL]. Its success can be largely attributed to the development of new strategies for high-throughput cloning and expression, and improved manufacturing techniques for the construction of arrays. Here, we describe a method to hybridize protein arrays with malaria patients' sera in order to identify seroreactive antigens, some of which may have a high potential of conferring protection from severe forms of malaria.

• Authors: Apoorva Venkatesh, Aarti Jain, Huw Davies, Philip L Felgner, Pradipsinh K Rathod, Swati Patankar, Sanjeeva Srivastava
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2344 Pg. 139-150 ( 2021) ISSN: 1940-6029 [Electronic] United States
• PMID: 34115357 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Protozoan Proteins

Topics

• Biomarkers (analysis)
• Humans
• Malaria (diagnosis, immunology)
• Protein Array Analysis
• Protozoan Proteins (analysis, immunology)
• Serologic Tests