Long noncoding RNA ZFPM2-AS1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the miR-576-3p/HIF-1α axis.

Abstract

Long noncoding RNA (LncRNA) zinc finger protein multitype 2 antisense RNA 1 (ZFPM2-AS1) is highly expressed in a variety of tumors and is involved in promoting the malignant biological behaviors of cancer cells. However, the mechanism of ZFPM2-AS1 in the progression of hepatocellular carcinoma (HCC) remains to be explored. The ZFPM2-AS1 expression in HCC was measured by quantitative real-time PCR (qRT-PCR); cell counting kit-8, 5-bromo-2'-deoxyuridine (BrdU), and transwell assays were used to confirm the biological functions of ZFPM2-AS1 in regulating the malignant biological behaviors of HCC cells; the luciferase reporter gene assay was employed to detect whether ZFPM2-AS1 could bind to microRNA (miR)-576-3p; the regulatory relationship between ZFPM2-AS1 and miR-576-3p was probed by qRT-PCR; the effects of ZFPM2-AS1 and miR-576-3p on the expression of hypoxia-inducible factor 1α (HIF-1α) were detected by qRT-PCR and Western blot. The expression of ZFPM2-AS1 in HCC tissues, compared with that in normal liver tissues, was significantly upregulated. Knockdown of ZFPM2-AS1 markedly inhibited HCC cell proliferation, migration, and invasion while the overexpression of ZFPM2-AS1 worked oppositely. miR-576-3p could reverse the effects of ZFPM2-AS1 on the biological behaviors of HCC cells. Besides, ZFPM2-AS1 could bind to miR-576-3p and positively regulate the expression of HIF-1α, a target gene of miR-576-3p, by adsorbing miR-576-3p. ZFPM2-AS1 is abnormally highly expressed in HCC and facilitates proliferation, migration, and invasion of HCC cells by adsorbing miR-576-3p and upregulating HIF-1α expression.

• Authors: Yubao Song, Xin Jin, Yong Liu, Shuiying Wang, Fang Bian, Qingqing Zhao, Hanping Shi, Zefeng Gao
• Journal: Anti-cancer drugs (Anticancer Drugs) Vol. 32 Issue 8 Pg. 812-821 (09 01 2021) ISSN: 1473-5741 [Electronic] England
• PMID: 34102651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.

Chemical References

• DNA-Binding Proteins
• Hypoxia-Inducible Factor 1, alpha Subunit
• MIRN576 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• Transcription Factors
• ZFPM2 protein, human

Topics

• Adult
• Aged
• Carcinoma, Hepatocellular (pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• DNA-Binding Proteins (pharmacology)
• Female
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (drug effects)
• Liver Neoplasms (pathology)
• Male
• MicroRNAs (drug effects)
• Middle Aged
• RNA, Long Noncoding (pharmacology)
• Transcription Factors (pharmacology)