Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis.

Abstract

Linkage between bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) co-exposure and type 2 diabetes mellitus (T2DM), as well as ability of multi-strained probiotic to reduce DEHP, DBP and BPA mixture-induced oxidative damage in rat pancreas were investigated. The Comparative Toxicogenomics Database, Cytoscape software and ToppGene Suite were used for data-mining. Animals were sorted into seven groups (n = 6): (1) Control group: corn oil, (2) P: probiotic: Saccharomyces boulardii + Lactobacillus rhamnosus + Lactobacillus plantarum LP 6595 + Lactobacillus plantarum HEAL9; (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day, and (6) MIX: 50 mg/kg b.w./day DEHP + 50 mg/kg b.w/day DBP + 25 mg/kg b.w./day BPA; (7) MIX + P. Rats were sacrificed after 28 days of oral exposure. In silico investigation highlighted 44 DEHP, DBP and BPA mutual genes linked to the T2DM, while apoptosis and oxidative stress were highlighted as the main mechanisms of DEHP, DBP and BPA mixture-linked T2DM. In vivo experiment confirmed the presence of significant changes in redox status parameters (TOS, SOD and SH groups) only in the MIX group, indicating possible additive effects, while probiotic ameliorated mixture-induced redox status changes in rat pancreatic tissue.

• Authors: Katarina Baralić, Katarina Živančević, Dragica Jorgovanović, Dragana Javorac, Jelena Radovanović, Tamara Gojković, Aleksandra Buha Djordjevic, Marijana Ćurčić, Zoran Mandinić, Zorica Bulat, Biljana Antonijević, Danijela Đukić-Ćosić
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 154 Pg. 112325 (Aug 2021) ISSN: 1873-6351 [Electronic] England
• PMID: 34097988 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier Ltd. All rights reserved.

Chemical References

• Benzhydryl Compounds
• Endocrine Disruptors
• Phenols
• Plasticizers
• Protective Agents
• Dibutyl Phthalate
• Diethylhexyl Phthalate
• bisphenol A

Topics

• Animals
• Apoptosis (drug effects)
• Benzhydryl Compounds (toxicity)
• Computational Biology
• Diabetes Mellitus, Type 2 (chemically induced, genetics, prevention & control)
• Dibutyl Phthalate (toxicity)
• Diethylhexyl Phthalate (toxicity)
• Endocrine Disruptors (toxicity)
• Gene Expression (drug effects)
• Male
• Oxidative Stress (drug effects)
• Pancreas (drug effects)
• Phenols (toxicity)
• Plasticizers (toxicity)
• Probiotics (therapeutic use)
• Protective Agents (therapeutic use)
• Rats
• Toxicogenetics