Abstract | PURPOSE: MATERIALS AND METHODS: We performed a wound-healing assay and a Transwell migration and invasion assay to assess the migration and invasion of prostate cancer cells. Western blot analysis was used to measure the expression of cathepsin B, MMP2, and MMP9. RESULTS: TRPV6 siRNA significantly inhibited the proliferation of LNCaP prostate cancer cells. It also significantly attenuated the wound healing and migration capacities of LNCaP cells. Moreover, the invasiveness of LNCaP cells and the expression of MMP9 and cathepsin B in LNCaP cells were also significantly inhibited by TRPV6 siRNA. CONCLUSIONS:
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Authors | Duk Yoon Kim, Soon Hee Kim, Eun Kyoung Yang |
Journal | Investigative and clinical urology
(Investig Clin Urol)
Vol. 62
Issue 4
Pg. 447-454
(07 2021)
ISSN: 2466-054X [Electronic] Korea (South) |
PMID | 34085788
(Publication Type: Journal Article)
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Copyright | © The Korean Urological Association, 2021. |
Chemical References |
- Calcium Channels
- RNA, Small Interfering
- TRPV Cation Channels
- TRPV6 protein, human
- CTSB protein, human
- Cathepsin B
- MMP2 protein, human
- Matrix Metalloproteinase 2
- MMP9 protein, human
- Matrix Metalloproteinase 9
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Topics |
- Calcium Channels
(genetics, metabolism)
- Cathepsin B
(metabolism)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Humans
- Male
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Prostatic Neoplasms
(genetics, metabolism, pathology)
- RNA Interference
- RNA, Small Interfering
- TRPV Cation Channels
(genetics, metabolism)
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