Measurement batch differences and between-batch conversion of Alzheimer's disease cerebrospinal fluid biomarker values.

Abstract

INTRODUCTION:

Batch differences in cerebrospinal fluid (CSF) biomarker measurement can introduce bias into analyses for Alzheimer's disease studies. We evaluated and adjusted for batch differences using statistical methods.

METHODS:

A total of 792 CSF samples from 528 participants were assayed in three batches for 12 biomarkers and 3 biomarker ratios. Batch differences were assessed using Bland-Altman plot, paired t test, Pitman-Morgan test, and linear regression. Generalized linear models were applied to convert CSF values between batches.

RESULTS:

We found statistically significant batch differences for all biomarkers and ratios, except that neurofilament light was comparable between batches 1 and 2. The conversion models generally had high R 2 except for converting P-tau between batches 1 and 3.

DISCUSSION:

Between-batch conversion allows harmonized CSF values to be used in the same analysis. Such method may be applied to adjust for other sources of variability in measuring CSF or other types of biomarkers.

• Authors: Yue Ma, Derek L Norton, Carol A Van Hulle, Richard J Chappell, Karen K Lazar, Erin M Jonaitis, Rebecca L Koscik, Lindsay R Clark, Rachel Krause, Ulf Andreasson, Nathaniel A Chin, Barbara B Bendlin, Sanjay Asthana, Ozioma C Okonkwo, Carey E Gleason, Sterling C Johnson, Henrik Zetterberg, Kaj Blennow, Cynthia M Carlsson
• Journal: Alzheimer's & dementia (Amsterdam, Netherlands) (Alzheimers Dement (Amst)) Vol. 13 Issue 1 Pg. e12194 ( 2021) ISSN: 2352-8729 [Print] United States
• PMID: 34084888 (Publication Type: Journal Article)
• Copyright: © 2021 The Authors. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring published by Wiley Periodicals, LLC on behalf of Alzheimer's Association.