Pinocembrin ameliorates arrhythmias in rats with chronic ischaemic heart failure.

Abstract

OBJECTIVE:

Ventricular arrhythmias (VAs) are a common complication of chronic ischaemic heart failure (CIHF). The purpose of this study is to investigate the efficacy of pinocembrin in a rat model of VAs induced by CIHF and further examine the possible mechanism.

METHODS:

Rats were subjected to ligation of left anterior descending coronary artery to mimic CIHF and then received pinocembrin treatment daily for 2 months. The vivo electrophysiology were performed to determine the effect of pinocembrin on ventricular electrical activity. The expression of Cav1.2, Kv4.2, and NGF was determined by Western blot. The structural change of ventricle was tested by the Echocardiography, Masson staining, and HE staining. The effect of pinocembrin on sympathetic nerve-related markers was detected by the immunostaining and the ELISA was used to test for biomarkers associated with heart failure.

RESULTS:

Pinocembrin increased the expression of ion channel protein Cav1.2 and Kv4.3, ameliorated the shortening of action potential duration (APD) and reduced the incidence and duration of ventricular fibrillation (VF). Pinocembrin also reduced the expression of nerve growth factor (NGF) and improved the autonomic nerve remodelling. In addition, pinocembrin reduced the area of infarct area and myocardial fibrosis, accompanied by increasing the expression of connexin protein 43 (CX43).

CONCLUSION:

We demonstrate that pinocembrin reduces cardiac nerve remodelling and protects against Vas induced by CIHF. The findings suggest that pinocembrin can be a promising candidate for the treatment of VAs.

• Authors: Yan Guo, Cui Zhang, Tianxin Ye, Xiuhuan Chen, Xin Liu, Xiaoli Chen, Yazhou Sun, Chuan Qu, Jinjun Liang, Shaobo Shi, Bo Yang
• Journal: Annals of medicine (Ann Med) Vol. 53 Issue 1 Pg. 830-840 (12 2021) ISSN: 1365-2060 [Electronic] England
• PMID: 34060948 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavanones
• pinocembrin
• Nerve Growth Factor

Topics

• Animals
• Arrhythmias, Cardiac (etiology, prevention & control)
• Disease Models, Animal
• Flavanones (therapeutic use)
• Heart Failure (drug therapy, etiology, prevention & control)
• Humans
• Myocardial Infarction
• Nerve Growth Factor
• Rats