S-Allylmercapto-N-acetylcysteine ameliorates elastase-induced chronic obstructive pulmonary disease in mice via regulating autophagy.

Abstract

Autophagy-impairment is involved in the pathological process of chronic obstructive pulmonary disease (COPD), and relates to inflammation and emphysema in lung injury. This study aimed to elucidate the protective effect of S-Allylmercapto-N-acetylcysteine (ASSNAC) against COPD via regulating the autophagy. Firstly, porcine pancreatic elastase (PPE)-induced COPD model in A549 cells was established, and ASSNAC was verified to alleviate the autophagy-impairment from the results of western blotting analysis of LC3BⅡ/Ⅰ and monodansylcadaverine (MDC) staining of autophagosome. Secondly, Balb/c mice were stimulated by PPE to induce the COPD model in vivo. The histological analysis of lung tissues presented that ASSNAC could alleviate the lung injury induced by PPE. Thirdly, the secretions of NO, TNF-α and IL-1β in serum and BALF were reduced by ASSNAC compared with the PPE group. Finally, the mechanism of therapeutic effects of ASSNAC against COPD through regulating the autophagy-impairment was clarified. That is, ASSNAC inhibits the phosphorylation of PI3K/Akt/mTOR signaling pathways. In a word, this research provides a reference for ASSNAC to be an effective drug for pulmonary diseases.

• Authors: Dandan Zheng, Jinglong Wang, Genju Li, Lulu An, Ying Qu, Qinxiu Zhang, Wenhui Ye, Xin Zhao, Zhongxi Zhao
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 562 Pg. 83-88 (07 12 2021) ISSN: 1090-2104 [Electronic] United States
• PMID: 34044325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Allyl Compounds
• Map1lc3b protein, mouse
• Microtubule-Associated Proteins
• S-allylmercapto-N-acetylcysteine
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases
• Pancreatic Elastase
• Acetylcysteine

Topics

• A549 Cells
• Acetylcysteine (analogs & derivatives, pharmacology, therapeutic use)
• Allyl Compounds (pharmacology, therapeutic use)
• Animals
• Autophagy (drug effects)
• Disease Models, Animal
• Humans
• Inflammation (pathology)
• Lung (drug effects, pathology)
• Mice
• Mice, Inbred BALB C
• Microtubule-Associated Proteins (metabolism)
• Pancreatic Elastase
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Pulmonary Disease, Chronic Obstructive (chemically induced, drug therapy, pathology)
• Swine
• TOR Serine-Threonine Kinases (metabolism)
• Up-Regulation (drug effects)
• Weight Loss (drug effects)