Common Marmoset Model of α-Synuclein Propagation.

Abstract

The propagation of assembled α-synuclein (αS) is key to understanding the pathological mechanisms of synucleinopathies such as Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy.Here we describe a nonhuman primate model of αS propagation using common marmosets (Callithrix jacchus) with an intracerebral injection of synthetic preformed αS fibrils. This protocol enables observation of the formation of phosphorylated αS pathology and its propagation three months after the injection.

• Authors: Masami Masuda-Suzukake, Aki Shimozawa, Masashi Hashimoto, Masato Hasegawa
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2322 Pg. 131-139 ( 2021) ISSN: 1940-6029 [Electronic] United States
• PMID: 34043199 (Publication Type: Journal Article)

Chemical References

• alpha-Synuclein

Topics

• Amino Acid Sequence
• Animals
• Brain (metabolism, pathology)
• Callithrix
• Disease Models, Animal
• Phosphorylation (physiology)
• Synucleinopathies (metabolism, pathology)
• alpha-Synuclein (metabolism)