The purpose of this study was to discuss the effects of an extract from the culture medium of Pseudomonas aeruginosa (P. aeruginosa) 2016NX1 (
chloroform extract of P. aeruginosa, CEPA) and its purified product
1-hydroxyphenazine on RAW264.7 cell
inflammation. Cell viability was evaluated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium
bromide (MTT) method. TNF-α production was determined by an ELISA method. The effects of CEPA and its purified product
1-hydroxyphenazine on cell morphology were investigated using an inverted microscope. Quantitative real-time PCR was performed to determine
mRNA expression levels. CEPA and
1-hydroxyphenazine had no obvious toxicity to cells when their concentrations were no more than 20 μg ml-1 and 5 μg ml-1, respectively. Both CEPA and
1-hydroxyphenazine suppressed the secretion of TNF-α and significantly reduced the
mRNA expression levels of TNF-α, IL-1β, and
IL-6. Both CEPA and
1-hydroxyphenazine inhibited M1 cell polarization after
lipopolysaccharide (LPS) stimulation. The results in this article lay a good foundation for the biopharmaceutical applications of CEPA and
1-hydroxyphenazine in the future. CEPA and
1-hydroxyphenazine had certain anti-inflammatory activity, and inhibited LPS-induced RAW264.7 cell
inflammation. Our findings suggest that CEPA and
1-hydroxyphenazine are potential chemicals with anti-inflammatory activity.