Abstract |
COVID-19 pandemic has resulted in an unprecedented global public health crisis. It is obvious that SARS-CoV-2 vaccine is needed to control the global COVID-19 public health crisis. Since obvious advantages including fast manufacturing speed, potent immunogenicity and good safety profile, six mRNA vaccines have been used to prevent SARS-CoV-2 infections in clinic with lipid nanoparticles (LNP) formulation via intramuscular injection. In this work, we first constructed RBD-encoding mRNA (RBD- mRNA) formulated in liposomes (LPX/RBD- mRNA) and investigated the influence of administration routes on the immunogenicity. LPX/RBD- mRNA can express RBD in vivo and successfully induced SARS-CoV-2 RBD specific antibodies in the vaccinated mice, which efficiently neutralized SARS-CoV-2 pseudotyped virus. Moreover, the administration routes were found to affect the virus neutralizing capacity of sera derived from the immunized mice and the types (Th1-type and Th2-type) of cellular immune responses. This study indicated that liposome-based RBD- mRNA vaccine with optimal administration route might be a potential candidate against SARS-CoV-2 infection with good efficacy and safety.
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Authors | Hai Huang, Caili Zhang, Shuping Yang, Wen Xiao, Qian Zheng, Xiangrong Song |
Journal | Journal of controlled release : official journal of the Controlled Release Society
(J Control Release)
Vol. 335
Pg. 449-456
(07 10 2021)
ISSN: 1873-4995 [Electronic] Netherlands |
PMID | 34029632
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021. Published by Elsevier B.V. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19 Vaccines
- Liposomes
- RNA, Messenger
- Spike Glycoprotein, Coronavirus
- Vaccines
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Topics |
- Animals
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19
- COVID-19 Vaccines
- Humans
- Liposomes
- Mice
- Mice, Inbred BALB C
- Pandemics
- RNA, Messenger
- SARS-CoV-2
- Spike Glycoprotein, Coronavirus
- Vaccines
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