Apostichopus japonicus Oligopeptide Induced Heterogeneity in the Gastrointestinal Tract Microbiota and Alleviated Hyperuricemia in a Microbiota-Dependent Manner.

Abstract

SCOPE:

This study aims to investigate the protective effect of Apostichopus japonicus oligopeptide (AJOP) on hyperuricemia, demonstrate the modulation of the gastrointestinal tract (GIT) microbiota, and clarify the underlying microbiota-dependent mechanism.

METHODS AND RESULTS:

Hyperuricemic mice treated with AJOP and subjected to corresponding fecal microbiota transplantation (FMT) are used to observe the beneficial effects of AJOP and microbiota. Gene transcriptions are measured using quantitative real-time PCR. The GIT (stomach, colon, cecum, and feces) microbiota is analyzed by 16S rDNA sequencing and the short-chain fatty acids are detected using GC-MS. Dietary administration of AJOP significantly alleviates hyperuricemia, regulates uric acid metabolism, inhibites the activation of the NLRP3 inflammasome and NF-κB-related signaling pathway, and restores m6A methylation levels. In addition, substantial heterogeneity is observed in GIT microbiota. Furthermore, FMT effectively alleviates hyperuricemia in mice by selectively regulating the corresponding pathways associated with AJOP treatment, indicating that the mechanism underlying the protective effects of AJOP is partly microbiota-dependent.

CONCLUSION:

This study demonstrates that AJOP exerts a protective effect on hyperuricemic mice by regulating uric acid metabolism, resulting in substantial heterogeneity among the GIT microbiota, thus mediating the beneficial effects in a microbiota-dependent manner.

• Authors: Chenyang Lu, Shasha Tang, Jiaojiao Han, Siqing Fan, Yumeng Huang, Zhen Zhang, Jun Zhou, Tinghong Ming, Ye Li, Xiurong Su
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 65 Issue 14 Pg. e2100147 (07 2021) ISSN: 1613-4133 [Electronic] Germany
• PMID: 34018696 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 Wiley-VCH GmbH.

Chemical References

• NF-kappa B
• Oligopeptides
• Plant Preparations
• Uric Acid

Topics

• Animals
• Fecal Microbiota Transplantation
• Gastrointestinal Microbiome (drug effects)
• Hyperuricemia (drug therapy)
• Male
• Mice
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Oligopeptides (pharmacology)
• Plant Preparations (pharmacology)
• Signal Transduction (drug effects)
• Stichopus (chemistry)
• Uric Acid (metabolism)