PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon.

Abstract

Yellow head virus (YHV) is a pathogen which causes high mortality in penaeid shrimp. Previous studies suggested that YHV enters shrimp cells via clathrin-mediated endocytosis. This research investigated the roles of clathrin adaptor protein 2 subunit β (AP-2β) from Penaeus monodon during YHV infection. PmAP2-β was continuously up-regulated more than twofold during 6-36 hpi. Suppression of PmAP2-β significantly reduced YHV copy numbers and delayed shrimp mortality. Quantitative RT-PCR revealed that knockdown of PmAP2-β significantly enhanced the expression level of PmSpätzle, a signaling ligand in the Toll pathway, by 30-fold at 6 and 12 hpi. Moreover, the expression levels of gene components in the Imd and JAK/STAT signaling pathways under the suppression of PmAP2-β during YHV infection were also investigated. Interestingly, anti-lipopolysaccharide factor isoform 3 (ALFPm3) was up-regulated by 40-fold in PmAP2-β knockdown shrimp upon YHV infection. In addition, silencing of PmAP2-β dramatically enhanced crustinPm1 expression in YHV-infected shrimp. Knockdown of ALFPm3 and crustinPm1 significantly reduced shrimp survival rate. Taken together, this work suggested that PmAP2-β-deficiency promoted the Toll pathway signalings, resulting in elevated levels of ALFPm3 and crustinPm1, the crucial antimicrobial peptides in defence against YHV.

• Authors: Thapanan Jatuyosporn, Pasunee Laohawutthichai, Premruethai Supungul, Rogerio R Sotelo-Mundo, Adrian Ochoa-Leyva, Anchalee Tassanakajon, Kuakarun Krusong
• Journal: Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 10534 (05 18 2021) ISSN: 2045-2322 [Electronic] England
• PMID: 34006863 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Vesicular Transport
• Toll-Like Receptors

Topics

• Adaptor Proteins, Vesicular Transport (genetics)
• Animals
• Gene Silencing
• Penaeidae (genetics, virology)
• Roniviridae (isolation & purification)
• Signal Transduction
• Toll-Like Receptors (metabolism)