Effect of Daikenchuto On Spontaneous Intestinal Tumors in ApcMin/+ Mice.

Abstract

Daikenchuto (TU-100) is herbal medicine which predominantly contains ginger, Japanese pepper, and ginseng. We investigated whether TU-100 can affect the composition of gut flora and intestinal tumor development using ApcMin/+ mice, a murine model of intestinal tumor. Bacterial 16S rRNA sequencing and short-chain fatty acid analysis were performed on faecal samples. Tumor number and size were analysed. Any change in gene expression of the tumor tissues was assessed by real-time PCR. Principal coordinate analysis (PCoA) showed that the faecal microbiota cluster of TU-100-fed mice was different from the microbiota of control mice. However, no significant difference was observed in the concentration of short-chain fatty acids, tumor number, and gene expression levels between the two groups. Our data showed that TU-100 can affect the intestinal environment; however, it does not contribute in tumor progression or inhibition in our setting.

• Authors: Lingling Kong, Namiko Hoshi, Daisuke Watanabe, Yasutaka Yamada, Eiichiro Yasutomi, Soichiro Adachi, Makoto Ooi, Yunlong Sui, Ryutaro Yoshida, Ryohei Sekimoto, Eri Tokunaga, Haruka Miyazaki, Yuna Ku, Haruka Takenaka, Tadao Kunihiro, Jun Inoue, Zibin Tian, Yuzo Kodama
• Journal: The Kobe journal of medical sciences (Kobe J Med Sci) Vol. 66 Issue 4 Pg. E139-E148 (Jan 04 2021) ISSN: 1883-0498 [Electronic] Japan
• PMID: 33994517 (Publication Type: Journal Article)

Chemical References

• Plant Extracts
• RNA, Ribosomal, 16S
• dai-kenchu-to

Topics

• Animals
• Feces
• Gastrointestinal Microbiome (drug effects, genetics)
• Herbal Medicine
• Intestinal Mucosa (drug effects)
• Intestinal Neoplasms (drug therapy, pathology)
• Mice
• Microbiota
• Panax
• Plant Extracts (pharmacology)
• RNA, Ribosomal, 16S
• Real-Time Polymerase Chain Reaction
• Zanthoxylum
• Zingiberaceae