Urinary microRNAs as non-invasive biomarkers for toxic acute kidney injury in humans.

Abstract	MicroRNAs in biofluids are potential biomarkers for detecting kidney and other organ injuries. We profiled microRNAs in urine samples from patients with Russell's viper envenoming or acute self-poisoning following paraquat, glyphosate, or oxalic acid [with and without acute kidney injury (AKI)] and on healthy controls. Discovery analysis profiled for 754 microRNAs using TaqMan OpenArray qPCR with three patients per group (12 samples in each toxic agent). From these, 53 microRNAs were selected and validated in a larger cohort of patients (Russell's viper envenoming = 53, paraquat = 51, glyphosate = 51, oxalic acid = 40) and 27 healthy controls. Urinary microRNAs had significantly higher expression in patients poisoned/envenomed by different nephrotoxic agents in both discovery and validation cohorts. Seven microRNAs discriminated severe AKI patients from no AKI for all four nephrotoxic agents. Four microRNAs (miR-30a-3p, miR-30a-5p, miR-92a, and miR-204) had > 17 fold change (p < 0.0001) and receiver operator characteristics area-under-curve (ROC-AUC) > 0.72. Pathway analysis of target mRNAs of these differentially expressed microRNAs showed association with the regulation of different nephrotoxic signaling pathways. In conclusion, human urinary microRNAs could identify toxic AKI early after acute injury. These urinary microRNAs have potential clinical application as early non-invasive diagnostic AKI biomarkers.
Authors	Fathima Shihana, Wilson K M Wong, Mugdha V Joglekar, Fahim Mohamed, Indika B Gawarammana, Geoffrey K Isbister, Anandwardhan A Hardikar, Devanshi Seth, Nicholas A Buckley
Journal	Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 9165 (04 28 2021) ISSN: 2045-2322 [Electronic] England
PMID	33911095 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Biomarkers MicroRNAs Viper Venoms Oxalic Acid Paraquat Glycine
Topics	Acute Kidney Injury (chemically induced, genetics, urine) Animals Biomarkers (urine) Glycine (analogs & derivatives, poisoning) Humans MicroRNAs (urine) Oxalic Acid (toxicity) Paraquat (poisoning) Reproducibility of Results Daboia Viper Venoms (poisoning) Glyphosate

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!