Enhanced Neuroprotective Effects of Epicatechin Gallate Encapsulated by Bovine Milk-Derived Exosomes against Parkinson's Disease through Antiapoptosis and Antimitophagy.

Abstract

Epicatechin gallate (ECG) is a main effective catechin widely existing in natural plants and food, with well-known health benefits. The present study first designed a new exosome-based delivery system for ECG and examined its neuroprotective effects on a rotenone (Rot)-induced Parkinson's disease (PD) model in vitro. Exosomes (Exo) were isolated from fresh bovine milk, and their average size was 85.15 ± 2.00 nm. ECG was encapsulated into Exo by a sonication method, and the loading efficiency of ECG-loaded exosomes (ECG-Exo) was 25.96 ± 0.45%. The neuroprotective effects of ECG-Exo were evaluated on Rot-induced SHSY5Y cells and compared with free ECG. Cell viability, cellular reactive oxygen species, apoptosis rate, and the expressions of caspase-3, Bax, Bcl-2, parkin, PINK1, and Atg5 were determined. Our results showed that Exo delivered ECG successfully into SHSY5Y cells and exhibited enhanced neuroprotective effects. ECG-Exo might inhibit SHSY5Y cell damage induced by Rot through antiapoptosis and antimitophagy.

• Authors: Siqi Luo, Xiuli Sun, Meng Huang, Qianhui Ma, Libo Du, Yan Cui
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 69 Issue 17 Pg. 5134-5143 (May 05 2021) ISSN: 1520-5118 [Electronic] United States
• PMID: 33890462 (Publication Type: Journal Article)

Chemical References

• Neuroprotective Agents
• Catechin
• epicatechin gallate

Topics

• Animals
• Apoptosis
• Catechin (analogs & derivatives, pharmacology)
• Cattle
• Exosomes
• Milk
• Neuroprotective Agents (pharmacology)
• Parkinson Disease (drug therapy)