Lower urinary tract symptoms (LUTS) are common among aging men. Since prostatic
inflammation is one of its etiologies, it is plausible that urinary metabolite and
protein biomarkers could be identified and used to diagnose
inflammation-induced LUTS. We characterized the urine metabolome and
proteome in a mouse model of bacterial-induced prostatic
inflammation. Mass Spectrometry (MS)-based multi-omics analysis was employed to discover urinary
protein and metabolite-based
biomarkers. The investigation of isobaric dimethylated
leucine (DiLeu) labeling on metabolites allowed metabolomics and proteomics analysis on the same liquid chromatography (LC)-MS platform. In total, 143
amine-containing metabolites and 1058 urinary
proteins were identified and quantified (data are available via ProteomeXchange with identifier PXD018023); among them, 14 metabolites and 168
proteins were significantly changed by prostatic
inflammation. Five metabolic pathways and four
inflammation-related biological processes were potentially disrupted. By comparing our findings with urinary
biomarkers identified in a mouse model of genetic-induced prostate
inflammation and with those previously found to be associated with LUTS in older men, we identified
creatine,
haptoglobin,
immunoglobulin kappa constant and
polymeric Ig receptor as conserved
biomarkers for prostatic
inflammation associated with LUTS. These data suggest that these putative
biomarkers could be used to identify men in which prostate
inflammation is present and contributing to LUTS.