A novel application of delayed-type hipersensitivity reaction to measure cellular immune response in SARS-CoV-2 exposed individuals.

Abstract

OBJECTIVE:

To understand the anti-virus adaptive immune response occurring during SARS-Cov-2 infection is necessary to have methods to investigate cellular and humoral components. The goal of this study has been to investigate the utility of a specific spike-DTH test using a coronavirus recombinant protein in COVID-19 patients.

METHODS:

DTH studies were performed by intradermal injection of a commercial recombinant spike protein from SARS-CoV-2 along with conventional serology studies.

RESULTS:

Fifty-one COVID-19 patients were studied showing 84,3% of concordance with spike-DTH and anti-RBD-IgG. Spike-DTH was superior to identify seven more COVID-19 individuals. A high specificity was found with no positive spike DTH reactions in the non-sick individuals. The skin test also showed more stable results over time while specific anti-RBD-IgG decreased gradually. Clinical severity groups also showed a progressive gradient of larger positive spike-DTH.

CONCLUSION:

Specific spike DTH test seems to be an easy method to study cell immune response.

• Authors: Yvelise Barrios, Andres Franco, Inmaculada Sanchez-Machin, Paloma Poza-Guedes, Ruperto Gonzalez-Perez, Victor Matheu
• Journal: Clinical immunology (Orlando, Fla.) (Clin Immunol) Vol. 226 Pg. 108730 (05 2021) ISSN: 1521-7035 [Electronic] United States
• PMID: 33865990 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier Inc. All rights reserved.

Chemical References

• Antibodies, Viral
• Immunoglobulin G
• Recombinant Proteins
• Spike Glycoprotein, Coronavirus
• spike protein, SARS-CoV-2

Topics

• Antibodies, Viral (blood)
• COVID-19 (immunology)
• COVID-19 Serological Testing (methods)
• Female
• Humans
• Hypersensitivity, Delayed (immunology)
• Immunity, Cellular
• Immunoglobulin G (blood)
• Injections, Intradermal
• Male
• Middle Aged
• Protein Domains
• Recombinant Proteins
• SARS-CoV-2 (genetics)
• Spike Glycoprotein, Coronavirus (immunology)