Glycoprotein VI (GPVI), the platelet immunoreceptor
tyrosine activating motif (ITAM) receptor for
collagen, plays a striking role on vascular integrity in animal models of
inflammation and
sepsis. Understanding ITAM-receptor signaling defects in humans suffering from
sepsis may improve our understanding of the pathophysiology, especially during disease onset. In a pilot study, platelets from 15 patients with
sepsis were assessed consecutively at day of admission, day 5 to 7, and the day of intensive care unit (ICU) discharge and subjected to comprehensive analyses by flow cytometry, aggregometry, and immunoblotting. Platelet function was markedly reduced in all patients. The defect was most prominent after GPVI stimulation with
collagen-related peptide. In 14 of 15 patients, GPVI dysfunction was already present at time of ICU admission, considerably before the critical drop in platelet counts.
Sepsis platelets failed to transduce the GPVI-mediated signal to trigger
tyrosine phosphorylation of
Syk kinase or LAT. GPVI deficiency was partially inducible in platelets of healthy donors through coincubation in whole blood, but not in plasma from patients with
sepsis. Platelet aggregation upon GPVI stimulation increased only in those patients whose condition ameliorated. As blunted GPVI signaling occurred early at
sepsis onset, this defect could be exploited as an
indicator for early
sepsis diagnosis, which needs to be confirmed in prospective studies.