Sixty male Wistar rats were randomly divided into control, model and
Danhong groups with 25 rats in each group. Intracerebral injection of autologous arterial blood was performed on model and
Danhong groups in order to establish
intracerebral hemorrhage model. Rats in the control group were given the same operation procedure without blood injection. After successfully establishing the
intracerebral hemorrhage model, the rats were given
Danhong (2ml/kg/d) through
intraperitoneal injection. Rats in the control and model groups were given the same amount of
normal saline respectively. The brain water content (BWC) and serum level of TNF-α,
IL-6 and NF-κB were measured in all groups at the time points of day 1, 3, 5, 7 and 9.
RESULTS: The neurological deficit score (
NDS) were not statistical different in days 1, 3 and 5 between the model and
Danhong group (P>0.05); However, on day 7 and 9 after modeling, the
NDS in the
Danhong group was significant lower than that of the Model group (P<0.05). The brain water content in the model and
Danhong groups were significantly elevated compared to control group (P<0.05). The brain water content was significant elevated after modeling in the model and
Danhong groups on day 3 and gradually decreased over the next 6 days.The brain water content was significantly higher in the model group for days 3 to 9 compared to the
Danhong group (P<0.05). Compared to the model group, the serum NF-κb was significantly lower in the
Danhong group for the time point of day 3 and 5 (P<0.05); However, compared to the model group, the serum TNF-α and
IL-6 levels in the
Danhong group were significantly lower for each time point (P<0.05). Conclusion
Danhong injection can reduce
cerebral edema in rats with
cerebral hemorrhage, and protect the brain nerve function. These effects may be related to its function of regulating serum TNF-α, NF-κB and
IL-6 expression.