Abstract |
Accumulating evidence indicates that an elevated ephrin-A1 expression is positively correlated with a worse prognosis in some cancers such as colon and liver cancer. The detailed mechanism of an elevated ephrin-A1 expression in a worse prognosis still remains to be fully elucidated. We previously reported that ADAM12-cleaved ephrin-A1 enhanced lung vascular permeability and thereby induced lung metastasis. However, it is still unclear whether or not cleaved forms of ephrin-A1 are derived from primary tumors and have biological activities. We identified the ADAM12-mediated cleavage site of ephrin-A1 by a Matrix-assisted laser desorption ionization mass spectrometry and checked levels of ephrin-A1 in the serum and the urine derived from the primary tumors by using a mouse model. We found elevated levels of tumor-derived ephrin-A1 in the serum and the urine in the tumor-bearing mice. Moreover, inhibition of ADAM-mediated cleavage of ephrin-A1 or antagonization of the EphA receptors resulted in a significant reduction of lung metastasis. The results suggest that tumor-derived ephrin-A1 is not only a potential biomarker to predict lung metastasis from the primary tumor highly expressing ephrin-A1 but also a therapeutic target of lung metastasis.
|
Authors | Katsuaki Ieguchi, Takeshi Tomita, Toshifumi Takao, Tsutomu Omori, Taishi Mishima, Isao Shimizu, Massimiliano Tognolini, Alessio Lodola, Takuya Tsunoda, Shinichi Kobayashi, Satoshi Wada, Yoshiro Maru |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 22
Issue 5
(Mar 01 2021)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 33804570
(Publication Type: Journal Article)
|
Chemical References |
- Ephrin-A1
- Receptor, EphA2
- ADAM12 Protein
|
Topics |
- ADAM12 Protein
(genetics, metabolism)
- Animals
- Capillary Permeability
- Carcinoma, Lewis Lung
(genetics, metabolism, pathology)
- Ephrin-A1
(genetics, metabolism)
- HEK293 Cells
- Humans
- Mice
- Mice, Inbred C57BL
- Neoplasm Metastasis
- Receptor, EphA2
(genetics, metabolism)
- Tumor Cells, Cultured
|