Long non-coding RNA LINC01137 contributes to oral squamous cell carcinoma development and is negatively regulated by miR-22-3p.

Abstract

PURPOSE:

Long noncoding RNAs (lncRNAs) are emerging as key regulators in cancer initiation and progression. LINC01137 is a recently identified lncRNA of which the functional role in the development of oral squamous cell carcinoma (OSCC) has not been determined yet.

METHODS:

We analyzed the expression of LINC01137 using a microarray-based OSCC gene expression dataset (GSE31056), and validated the results obtained using RT-qPCR in 26 pairs of primary OSCC tumor tissues and adjacent non-tumor tissues. The proliferative and invasive effects of LINC01137 on OSCC cells were determined using CCK-8, colony formation and transwell assays, respectively. Targeted binding between miR-22-3p and LINC01137 was verified using a dual luciferase reporter assay.

RESULTS:

We found that LINC01137 was significantly upregulated in primary OSCCs. LINC01137 knockdown inhibited OSCC cell proliferation, migration and invasion, whereas LINC01137 overexpression induced opposite effects. LINC01137 upregulation along with p53 inhibition enhanced the malignant transformation of oral cells. In addition, we found that miR-22-3p can directly target LINC01137 through interaction with a putative miR-22-3p-binding site present within the LINC01137 sequence. A significant negative correlation was observed between LINC01137 and miR-22-3p expression in primary OSCC specimens. Exogenous overexpression of miR-22-3p markedly reduced the endogenous expression level of LINC01137 in OSCC cells. Additional functional assays showed that miR-22-3p overexpression enhanced the inhibitory effect of siRNA-mediated LINC01137 silencing on OSCC cell proliferation, migration and invasion, whereas miR-22-3p inhibition had the opposite effect.

CONCLUSIONS:

Our results indicate that LINC01137 functions as an oncogenic lncRNA in OSCC. miR-22-3p can directly target LINC01137 and negatively regulate its expression and function.

• Authors: Yong Du, Haiyan Yang, Yue Li, Wenli Guo, Yufeng Zhang, Haitao Shen, Lingxiao Xing, Yuehong Li, Wenxin Wu, Xianghong Zhang
• Journal: Cellular oncology (Dordrecht) (Cell Oncol (Dordr)) Vol. 44 Issue 3 Pg. 595-609 (Jun 2021) ISSN: 2211-3436 [Electronic] Netherlands
• PMID: 33797737 (Publication Type: Journal Article)

Chemical References

• MIRN22 microRNA, human
• MicroRNAs
• RNA, Long Noncoding

Topics

• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• MicroRNAs (genetics)
• Mouth Neoplasms (genetics, pathology)
• RNA, Long Noncoding (genetics)
• Squamous Cell Carcinoma of Head and Neck (genetics, pathology)