A Photopolymerized Semi-Interpenetrating Polymer Networks-Based Hydrogel Incorporated with Nanoparticle for Local Chemotherapy of Tumors.

Abstract

PURPOSE:

To address the issue of local drug delivery in tumor treatment, a novel nanoparticle-hydrogel superstructure, namely semi-interpenetrating polymer networks (semi-IPNs) hydrogel composed of poly (ethylene glycol) diacrylate (PEGDA) and hyaluronic acid (HA) and incorporated with paclitaxel (PTX) loaded PLGA nanoparticles (PEGDA-HA/PLGA-PTX), was prepared by in situ UV photopolymerization for the use of local drug delivery.

METHODS:

Using the gelation time, swelling rate and degradation rate as indicators, the optimal proportion of Irgacure 2959 initiator and the concentration of HA was screened and obtained for preparing hydrogels. Next, paclitaxel (PTX) loaded PLGA nanoparticles (PLGA-PTX NPs) were prepared by the emulsion solvent evaporation method.

RESULTS:

The mass ratio of the initiator was 1%, and the best concentration of HA was 5 mg/mL in PEGDA-HA hydrogel. In vitro experiments showed that PLGA-PTX NPs had similar cytotoxicity to free PTX, and the cell uptake ratio on NCI-H460 cells was up to 96% by laser confocal microscopy and flow cytometry. The drug release of the PEGDA-HA/PLGA-PTX hydrogel local drug delivery system could last for 13 days. In vivo experiments proved that PEGDAHA/PLGA-PTX hydrogel could effectively inhibit the tumor growth without causing toxic effects in mice.

CONCLUSIONS:

This study demonstrated that the PEGDA-HA/PLGA-PTX hydrogel is a promising local drug delivery system in future clinical applications for tumor therapy. A photopolymerized semi-interpenetrating polymer networks-based hydrogel incorporated with paclitaxel-loaded nanoparticles was fabricated by in situ UV photopolymerization, providing a promised nanoplatform for local chemotherapy of tumors.

• Authors: Yeying Wang, Qilong Li, Jing-E Zhou, Jingwen Tan, Minghao Li, Nan Xu, Feng Qu, Jian Chen, Ji Li, Jing Wang, Zhiqiang Liang, Lei Yu, Yiting Wang, Zhiqiang Yan
• Journal: Pharmaceutical research (Pharm Res) Vol. 38 Issue 4 Pg. 669-680 (Apr 2021) ISSN: 1573-904X [Electronic] United States
• PMID: 33796952 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Delayed-Action Preparations
• Drug Carriers
• Hydrogels
• poly(ethylene glycol)diacrylate
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyethylene Glycols
• Hyaluronic Acid
• Paclitaxel

Topics

• Animals
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacokinetics)
• Cell Line, Tumor
• Delayed-Action Preparations (administration & dosage, pharmacokinetics)
• Drug Carriers (chemistry)
• Drug Compounding (methods)
• Drug Liberation
• Humans
• Hyaluronic Acid (chemistry)
• Hydrogels (chemistry)
• Mice
• Nanoparticles (chemistry)
• Neoplasms (drug therapy, pathology)
• Paclitaxel (administration & dosage)
• Polyethylene Glycols (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer (chemistry)
• Xenograft Model Antitumor Assays