Plasma exosomal miR-596: a novel biomarker predicts survival in patients with idiopathic pulmonary artery hypertension.

Abstract

OBJECTIVE:

To determine if plasma exosomal microRNAs (miRNAs) can predict survival in patients with idiopathic pulmonary arterial hypertension (IPAH).

METHODS:

The study enrolled patients with IPAH that underwent right heart catheterization. Plasma was collected and exosomal miRNAs were extracted. Exosomes were evaluated using transmission electron microscopy, Western blot analysis and particle size distribution analysis. MiRNAs were evaluated using a miRNA microarray and validated using real-time polymerase chain reaction.

RESULTS:

This study included 12 patients with IPAH in the study group and 48 patients with IPAH in the validation group. The mean ± SD follow-up duration was 60.3 ± 35.4 months in the overall cohort. The levels of miR-596 were higher in the nonsurvivors compared with the survivors. The levels of miR-596 significantly correlated with survival time, mean right atrial pressure, pulmonary vascular resistance (PVR) and cardiac index. High levels of miR-596 and PVR were significantly associated with poor overall survival. Multivariate analysis demonstrated that exosomal miR-596 (hazard ratio [HR] = 2.119; 95% confidence interval [CI] 1.402, 3.203) and PVR (HR = 1.146; 95% CI 1.010, 1.300) were independent predictors of survival.

CONCLUSIONS:

High levels of plasma exosomal miR-596 were significantly associated with disease severity and poor prognosis of patients with IPAH.

• Authors: Yang Huang, Zuo-Gang Wang, Liang Tang, Su-Gang Gong, Yuan-Yuan Sun, Lan Wang, Rong Jiang, Wen-Hui Wu, Ci-Jun Luo, Jie Zhang, Xiao-Jun Yang, Jin-Ling Li, Xun-Tao Yuan, Qin-Hua Zhao, Ping Yuan
• Journal: The Journal of international medical research (J Int Med Res) Vol. 49 Issue 3 Pg. 3000605211002379 (Mar 2021) ISSN: 1473-2300 [Electronic] England
• PMID: 33788649 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• MIRN596 microRNA, human
• MicroRNAs

Topics

• Biomarkers
• Familial Primary Pulmonary Hypertension
• Humans
• Hypertension
• MicroRNAs (genetics)
• Prognosis
• Pulmonary Artery