Down-regulation of long non-coding RNA antisense non-coding RNA in the INK4 locus suppresses OVCAR-3 cells proliferation and induction of apoptosis by Wnt/β-catenin.

Abstract

OBJECTIVES:

Ovarian cancer is a lethal gynecological malignancy. Long non-coding RNA antisense non-coding RNA in the INK4 locus (lncRNA ANRIL) was reported to have a critical role in cancer advancement. The ANRIL-mediated oncogenic underlying molecular mechanisms are not fully understood in ovarian cancer. We aimed to study ANRIL silencing effects on the proliferation and apoptosis of OVCAR-3 cells.

METHODS:

The ANRIL was Knockdown by transfection of OVCAR-3 cells with si-RNA against ANRIL. MTT assay and cell death ELISA kit were used to evaluate cellular proliferation and apoptosis. The expression levels of ANRIL, pro-and anti-apoptotic genes were assessed using q-RT-PCR. Western blotting was used to assess Wnt/β-catenin signalling pathway.

KEY FINDINGS:

ANRIL down-regulating in OVCAR-3 cell lines resulted in significant inhibition of cellular proliferation, apoptosis induction, as well as suppression of cellular invasion. Besides, knockdown of ANRIL led to pro-apoptotic genes up-regulation, Bad and Bax and anti-apoptotic genes down-regulation, Bid and Bcl-2. More importantly, we observed that ANRIL inhibition suppressed the vital components expression of the Wnt/β-catenin cascade.

CONCLUSION:

Our findings showed that down-regulation of lncRNA ANRIL resulted in the effective suppression of OVCAR-3 cell proliferation and invasion and induction of apoptosis by preventing Wnt/β-catenin signal transduction.

• Authors: Lingna Sun, Yuping Cui, Kongdi Jiang, Juan Li
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 73 Issue 9 Pg. 1212-1217 (Aug 12 2021) ISSN: 2042-7158 [Electronic] England
• PMID: 33772549 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• BCL2 protein, human
• CDKN2B antisense RNA, human
• CTNNB1 protein, human
• MicroRNAs
• Proto-Oncogene Proteins c-bcl-2
• RNA, Long Noncoding
• Wnt Proteins
• beta Catenin

Topics

• Apoptosis
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs
• Neoplasm Invasiveness
• Ovarian Neoplasms (genetics, metabolism)
• Ovary (metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• RNA, Long Noncoding (metabolism)
• Signal Transduction
• Wnt Proteins (metabolism)
• beta Catenin (metabolism)