Regulation of translation by methylation multiplicity of 18S rRNA.

Abstract

N6-methyladenosine (m6A) is a conserved ribonucleoside modification that regulates many facets of RNA metabolism. Using quantitative mass spectrometry, we find that the universally conserved tandem adenosines at the 3' end of 18S rRNA, thought to be constitutively di-methylated (m62A), are also mono-methylated (m6A). Although present at substoichiometric amounts, m6A at these positions increases significantly in response to sulfur starvation in yeast cells and mammalian cell lines. Combining yeast genetics and ribosome profiling, we provide evidence to suggest that m6A-bearing ribosomes carry out translation distinctly from m62A-bearing ribosomes, featuring a striking specificity for sulfur metabolism genes. Our work thus reveals methylation multiplicity as a mechanism to regulate translation.

• Authors: Kuanqing Liu, Daniel A Santos, Jeffrey A Hussmann, Yun Wang, Benjamin M Sutter, Jonathan S Weissman, Benjamin P Tu
• Journal: Cell reports (Cell Rep) Vol. 34 Issue 10 Pg. 108825 (03 09 2021) ISSN: 2211-1247 [Electronic] United States
• PMID: 33691096 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Culture Media
• RNA, Ribosomal, 18S
• Methionine
• N-methyladenosine
• Adenosine

Topics

• Adenosine (analogs & derivatives, metabolism)
• Animals
• Cell Line
• Culture Media (chemistry)
• Humans
• Methionine (deficiency, metabolism)
• Methylation
• Mice
• Mutagenesis, Site-Directed
• Protein Biosynthesis (genetics)
• RNA, Ribosomal, 18S (genetics, metabolism)
• Ribosomes (metabolism)
• Saccharomyces cerevisiae (metabolism)