Abstract | OBJECTIVE: DESIGN: Laboratory studies. SETTING: Translational science laboratory. PATIENT(S)/ANIMAL(S): Human fibroid cell lines and patient-matched fibroid and myometrial cell lines. INTERVENTION(S): MAIN OUTCOME MEASURE(S): RESULT(S): CONCLUSION(S): Cells cultured on mechanically stiff substrates had enhanced PRB activation via a mechanism that required MEK 1/2 and AKAP13/RhoA/ROCK signaling pathways. These studies provide a framework to explore the mechanisms by which mechanical stiffness affects progesterone receptor activation.
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Authors | Christina N Cordeiro Mitchell, Md Soriful Islam, Sadia Afrin, Joshua Brennan, Kevin J Psoter, James H Segars |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 116
Issue 1
Pg. 255-265
(07 2021)
ISSN: 1556-5653 [Electronic] United States |
PMID | 33676751
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020. Published by Elsevier Inc. |
Chemical References |
- Ligands
- Polystyrenes
- Protein Kinase Inhibitors
- Receptors, Progesterone
- Silicones
- progesterone receptor B
- Progesterone
- MAP2K2 protein, human
- rho-Associated Kinases
- MAP Kinase Kinase 1
- MAP Kinase Kinase 2
- MAP2K1 protein, human
- rho GTP-Binding Proteins
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Topics |
- Cell Culture Techniques
- Cell Line, Tumor
- Extracellular Matrix
(genetics, metabolism, pathology)
- Female
- Humans
- Leiomyoma
(enzymology, genetics, pathology)
- Ligands
- MAP Kinase Kinase 1
(antagonists & inhibitors, metabolism)
- MAP Kinase Kinase 2
(antagonists & inhibitors, metabolism)
- Mechanotransduction, Cellular
(drug effects)
- Polystyrenes
(chemistry)
- Progesterone
(pharmacology)
- Protein Kinase Inhibitors
(pharmacology)
- Receptors, Progesterone
(agonists, metabolism)
- Silicones
(chemistry)
- Uterine Neoplasms
(enzymology, genetics, pathology)
- rho GTP-Binding Proteins
(antagonists & inhibitors, metabolism)
- rho-Associated Kinases
(antagonists & inhibitors, metabolism)
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