A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma.
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| Abstract |
Herein we report a quantitative, multiplex assay for disease markers in plasma based on an integrated setup of a portable scanner and a disposable paper-based analytical device (PAD). The quantitative analysis relies on the digital colorimetric reading of the three-layer PAD with 30 assay sites for performing respective chromogenic reactions for plasma uric acid, glucose, and triglyceride, which are considered as important risk factors for cardiovascular diseases. A portable scanner with WiFi transmission capability was used to produce high-quality color images of the PADs and wirelessly transfer them to a smartphone or other mobile devices for data processing. The concentrations of biomarkers in both standard solutions and plasma samples can be directly obtained using a custom-designed smartphone app that is also capable of constructing calibration curves. The detection limits of uric acid, glucose, and triglyceride were determined to be 0.50 mg/dL, 0.84 mmol/L, and 14 mg/dL, respectively, which are below the normal limits and adequate for clinical validation. Owing to the distinct advantages-simple, portable, and cost-effective-this mobile assay protocol can be used for point-of-care (POC) settings or resource-limited situations, and potentially for the diagnosis and prevention of infectious diseases.
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| Authors | Pengfei Hou, Rong Deng, Jiqiang Guo, Weiyi Chen, Xiaochun Li, Hua-Zhong Yu |
| Journal | Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 413 Issue 18 Pg. 4625-4634 (Jul 2021) ISSN: 1618-2650 [Electronic] Germany |
| PMID | 33661349 (Publication Type: Journal Article) |
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