Long non-coding RNAs (lncRNAs) are crucial in controlling important aspects of
tumor immunity. However, whether the expression pattern of lncRNAs in stomach
adenocarcinoma (STAD) reflects
tumor immunity is not fully understood. We screened differentially expressed lncRNAs (DElncRNAs) between high and low
tumor mutation burden (TMB) STAD samples. Using the least absolute shrinkage and selection operator method, 33 DElncRNAs were chosen to establish a
lncRNA-based signature classifier for predicting TMB levels. The accuracy of the 33-lncRNA-based signature classifier was 0.970 in the training set and 0.950 in the test set, suggesting the expression patterns of the 33 lncRNAs may be an
indicator of TMB in STAD. Survival analysis showed that a lower classifier index reflected better prognosis for STAD patients, and the index showed correlation with expression of
immune checkpoint molecules (PD1, PDL1, and CTLA4), tumor-infiltrating lymphocytes, and
microsatellite instability. In conclusion, STAD samples with different
tumor mutation burdens have different
lncRNA expression patterns. The 33-lncRNA-based signature classifier index may be an
indicator of TMB and is associated expression of immune checkpoints, tumor-infiltrating lymphocytes, and
microsatellite instability.