Abstract | OBJECTIVE: METHODS: Endogenous expression levels of circRNA_100395 and microRNA-1228 (miR-1228) in prostate cancer tissue samples and cell lines were detected by quantitative reverse transcription-polymerase chain reaction. Cell proliferation, invasion, and migration, cell cycle distribution, and epithelial-mesenchymal transition (EMT) were analyzed in circRNA_100395-overexpressing prostate cancer cells by Cell Counting Kit-8, flow cytometry, Transwell assay, and western blotting, respectively. RESULTS:
CircRNA_100395 expression was downregulated in cancerous prostate tissues relative to adjacent normal tissues. CircRNA_100395 expression was negatively correlated with tumor size, Gleason score, tumor stage, and lymph node metastasis. Moreover, circRNA_100395 overexpression inhibited cell proliferation, altered cell cycle distribution, reduced cell migration and invasion abilities, and suppressed EMT in prostate cancer cells. Moreover, miR-1228 was a direct downstream target of circRNA_100395, and the anti- tumor ability of circRNA_100395 was significantly reversed by miR-1228. CONCLUSION:
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Authors | Haitian He, Jianhua Li, Mayao Luo, Qiang Wei |
Journal | The Journal of international medical research
(J Int Med Res)
Vol. 49
Issue 2
Pg. 300060521992215
(Feb 2021)
ISSN: 1473-2300 [Electronic] England |
PMID | 33641485
(Publication Type: Journal Article)
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Chemical References |
- MIRN1228 microRNA, human
- MicroRNAs
- RNA, Circular
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Topics |
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
(genetics)
- Humans
- Male
- MicroRNAs
(genetics)
- Prostatic Neoplasms
(genetics)
- RNA, Circular
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