[Effects of Apelin-13 on barrier injury of human umbilical vein endothelial cells induced by LPS].

Abstract

Objective: To investigate the effects of Apelin-13 on barrier function injury of human umbilical vein endothelial cells (HUVECs) induced by LPS. Methods: The HUVECs cultured in vitro were divided into 4 groups: Control group, LPS group, Apelin-13+LPS group, Apelin-13 group. HUVECs were treated by 5 μg/ml LPS for 24 h to replicate the model with endothelial barrier impaired. Apelin-13 at the concentration of 1 μmol/L was given 30 min before LPS treatment. The cell viabillity of HUVECs was measured by CCK-8 assay. Protein expressions of VE-cadherin and F-actin were measured by Western blot and immunofluorescence. Nuclear factor κB p65(NF-κB p65) was detected by immunofluorescence. Results: Compared with the control group, the cell viabillity of HUVECs and protein expression of VE-cadherin were decreased by LPS, but the protein expression of F-actin and activation of NF-κB p65 were increased by LPS. These effects were attenuated by Apelin-13 administration. Conclusion: Apelin-13 ameliorates LPS-induced barrier function injury of HUVECs, which may be related to the inhibition of inflammation.

• Authors: Dao-Peng Lin, Sha Chen, Li-Ling Lu, Yu Wang, Fang-Fang Lyu, Lin-Wei Shi, Xiao-Xia Kong, Xiao-Ou Shan, Hai-Lin Zhang
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 36 Issue 5 Pg. 390-393 (Sep 2020) ISSN: 1000-6834 [Print] China
• PMID: 33629549 (Publication Type: Journal Article)

Chemical References

• Intercellular Signaling Peptides and Proteins
• Lipopolysaccharides
• NF-kappa B
• apelin-13 peptide

Topics

• Cells, Cultured
• Human Umbilical Vein Endothelial Cells
• Humans
• Intercellular Signaling Peptides and Proteins
• Lipopolysaccharides
• NF-kappa B