C-C motif
chemokine 18 (CCL18) belongs to the
chemokine CC family and is predominantly secreted by M2-tumor-associated macrophages. It has been reported to be associated with various diseases and
malignancies. Previous studies showed that CCL18 promotes
metastasis by activating downstream
kinases. However, it remains unknown whether CCL18 regulates post-translational modifications, other than phosphorylation, during
tumorigenesis. Here, we demonstrate that CCL18 is up-regulated in
non-small cell lung cancer (NSCLC) and is involved in regulating the
lysine acetylome in A549 cells. Using the combination of SILAC labeling and high-efficiency acetylation enrichment methods, we identified 1372
lysine acetylation (Kac) sites on 796
proteins in CCL18-treated A549 cells. Among the identified Kac sites, 147 from 126
proteins were down-regulated and seven from five
proteins were up-regulated with fold changes more than two and the p-value less than 0.05. Bioinformatics analysis further showed that the
proteins with down-regulated acetylation play critical roles in glycolysis, oxidative phosphorylation, tricarboxylic acid cycle, and pentose phosphate pathway in A549 cells. These results suggest that CCL18 may be involved in the development of NSCLC by regulating acetylation of the
proteins in many fundamental cellular processes, especially the metabolic reprogramming of
tumor cells.